TALEN相关论文
由于猪的诸多生理和遗传特征与人类近似，猪一直被认为是理想的异种器官移植候选供体动物。但是物种之间严重的免疫不相容是影响猪到......
基因编辑是进行生物体遗传修饰的重要手段,已广泛应用于功能基因组学研究、动物模型制备、动植物遗传改良、基因治疗等多个研究领......
转录激活样效应因子（transcription activator-like effector,TALE）作为一种强有力的基因调控和基因组编辑工具,由于繁琐耗时的构建......
由于猪的诸多生理和遗传特征与人类近似,猪一直被认为是理想的异种器官移植候选供体动物.但是物种之间严重的免疫不相容是影响猪到......
基因编辑是对基因进行靶向的和有目的的编辑,随着基因编辑技术的发展,基因编辑在疾病治疗中的应用也越来越广。本文主要介绍基因编......
期刊
先天性心脏病（congenital heart disease,CHD）是常见的出生缺陷,其病因是胚胎期心脏发育调控异常。先天性左室流出道梗阻（left ventri......
羊乳是一种理想的牛乳过敏症患者的替代乳品,TALEN和CRISPR/Cas9基因编辑技术是羊乳“人源化”改造的两大利器,能够与核酸内切酶相......
期刊
...
Somatic cell nuclear transfer (SCNT) has valuable application in the swine industry as well as bioscience research, but ......
罗非鱼是世界性养殖鱼类,雄鱼较雌鱼生长快50％,因此,其性别决定和性别控制的研究一直是国际前沿和热点.TALEN和CRISPR/Cas9是当前最......
会议
Objective—Vascular endothelial growth factor A(VEGFA)regulates both vasculogensis/angiogenesis and heart development.We......
Construction of TALEN Plasmids and The General Vector of Multiple Locus Gene Targeting of Human Cell
Construction of TALEN plasmids to knock out rDNA and the general vector of multiple locus gene targeting.Using online so......
目的：动物性状特别是多基因性状的改良需要多基因联合干预。针对人类疾病的靶向药物治疗十分有效，那么针对其靶点的基因干预治疗效果......
会议
Generation of TALE activators and TAL repressors to “knock up” or knock down gene expressions in zeb
Transcription Activator-like Effector Nuclease(TALEN)has been proven to be an effective tool for genome editing.It is co......
Heritable Targeted Inactivation of myostatin b Gene In Yellow Catfish(Tachysurus fulvidraco)using tr
Myostatin(Mstn)is a negative regulator of mammalian muscle growth.Though it has duplicated copies of mstn in fish,little......
Purpose Congenital cataract results from mutations that leading to severely disruption in the lens cell organization.EZR......
基因敲除动物模型一直以来是在活体动物上开展基因功能研究、寻找合适药物作用靶标的重要工具。传统的基因敲除方法需要通过复......
会议
Moyamoya disease(MMD)is characterized by a stenosis at the terminal of the internal carotid artery and an abnormal v......
精子发生涉及许多基因的表达调控。脊椎动物中,piwi缺失引起精子发育异常,导致雄性不育。配子母细胞特异因子1(Gtsf1)属未知蛋......
RP2基因突变导致X 连锁视网膜色素变性,可引起患者视力渐进性丧失并最终失明,是最为严重的视网膜色素变性类型之一.RP2 在视网......
Zebrafish is an excellent model for circadian studies.Lack of mutants for circadian clock genes,however,has hindered......
There are six cry genes in zebrafish.Among them,cry1a acts as a repressor in the circadian clock feedback loop.Here ......
As a zinc finger transcription factor,Sp7 is expressed specifically in osteoblasts.Because Sp7 knockout mice are emb......
Antagonistic roles of dmrt1 and foxl2 in sex differentiation via estrogen production in tilapia as d
Transcription activator-like effector nucleases (TALENs) are a powerful approach for targeted genome editing and ha......
减数分裂是有性生殖的个体在形成生殖细胞过程中发生的一种特殊分裂方式.研究表明视黄酸(retinoic acid,RA)信号通路在哺乳类,......
Gene editing in non-human primates may lead to valuable models for exploring the aetiologies and therapeutic strateg......
转录激活因子样效应物核酸酶(Transcription activator-like effector nucleases,TALEN)是目前最有发展前景的基因组修饰技术.......
Arl4aa is required for initiation of definitive hematopoiesis by maintaining Golgi complex integrity
ADP-ribosylation Factor Like 4aa(arl4aa)is a member of ADP ribosylation factor(ARF)family,a group of GTPases control......
人类胚胎干细胞(hESC)和诱导性干细胞(iPSC)具有体外无限扩增和分化成各种组织的能力，是研究疾病和发育的重要模型。在这两种细......
ObjectiveTo develop mouse colitis model by inactivating ACE2 gene via TALEN technology.Methods A pair of TALENs targ......
基因组编辑是指对基因组进行定点修饰的技术,在进行基因的功能研究、基因精准治疗以及由基因突变引起的重大疾病临床研究中具有重......
会议
PRMT5是一种蛋白质精氨酸甲基转移酶,在核糖体生物合成、高尔基体的组装、细胞生长、细胞增殖、细胞凋亡、细胞分化、细胞全能性及......
研究背景CD99 antigen-like 2(CD99L2)是一种高度糖基化的Ⅰ型跨膜蛋白,不属于任何已知的蛋白家族。CD99L2蛋白分布广泛,在人和小......
VEGF(Vascular Endothelial Growth Factor,VEGF)家族共分为六个亚组,包括血管内皮细胞生长因子A、B、C、D、E及胎盘生长因子。其......
水稻的耐盐碱性质一直是各大育种科学家们研究的重点之一。本研究利用类转录激活因子效应物核酸酶（Transcription activator-like e......
目的:Fascin2(Fscn2)是一种肌动蛋白交联蛋白,主要表达于视网膜和毛细胞静纤毛。最近的研究证实Fascin2基因(Fscn2)错义突变(Fasci......
热休克转录因子（Heat shock transcription factors,HSFs）或称为热休克因子,广泛存在于真核生物细胞中,是在功能和结构上同源性很高......
生物钟是生物体内的计时机制,在生理、生化和行为等水平调节生物体的生命活动。生物钟的异常可以导致失眠、精神障碍甚至癌症等许......
传统的基因组修饰技术中常用的是同源重组和逆转录病毒介导法，由于这些方法存在效率低、特异性差、病毒整合到基因组等问题，制约了其......
类转录激活因子效应物核酸酶（Transcription activator-like effector nuclease，TALEN）技术是近几年发展起来的一种可对基因组进行定......
目的:在肝脏L-02 SIRT6-KO细胞系中,观察SIRT6在肝脏脂滴形成中的作用,初步研究在肝脏脂质代谢中SIRT6相关的转录差异基因发挥作用......
基因组编辑是指对基因组进行定点修饰的技术,在进行基因的功能研究、基因精准治疗以及由基因突变引起的重大疾病临床研究中具有重......
期刊
,Combining 53BP1 with BRCA1 as a biomarker to predict the sensitivity of poly(ADP-ribose) polymerase
Over half of patients with BRCA1-deficient cancers do not respond to treatment with poly(ADP-ribose) polymerase (PARP) i......
,Genome editing in Bombyx mori: New opportunities for silkworm functional genomics and the sericultu
In recent years,research in life sciences has been remarkably revolutionized owing to the establishment,development and ......
